Professor Mattew 'Matt' Matic / Lieutenant Ninety / Zoonie the Lazoon / Doctor Baker (voice)

Jimmy Gibson (voice) (uncredited)

Colonel Steve Zodiac (voice)

Robert the Robot (voice) (uncredited)

Commander Wilbur Zero / Jock Campbell (voice)

Mike Mercury / Film Narrator (voice)

Professor Rudolph Popkiss (voice) (credit only)

Sheriff Tex Tucker / Telegraph Operator Dan Morse (voice)

Dusty the Dog / Rocky the Horse / Foreman (voice)

Martha 'Ma' Jones (voice)

Sheriff Tex Tucker (singing voice) (uncredited)