Peter Parker / Spider-Man (voice)

Mary Jane Watson (voice)

Doctor Octopus (voice)

Norman Osborn / Green Goblin (voice)

Robert Farrell / Rocket Racer (voice)

J. Jonah Jameson (voice)

Harry Osborn (voice)

Madame Web (voice)

Anastasia Hardy (voice)

Aunt May (voice)

Felicia Hardy (voice)

Jackson Weele (voice)

Anna Watson (voice)

Joe 'Robbie' Robertson (voice)

Liz Allen (voice)

Flash Thompson (voice)

Maria Taina Elizando (voice)

Doctor Farley Stillwell (voice)

Wilson Fisk / Kingpin (voice)

Mousie (voice)

Doctor Michael Pingree (voice)

Wardell Stromm (voice)

Detective Terri Lee (voice)

Mrs. Farrell (voice)

Jason Phillips (voice)