Peter Parker/Spider-Man (voice)

Gwen Stacy (voice)

Harry Osborn (voice)

Norman Osborn (voice)

Aunt May Parker (voice)

Eddie Brock (voice)

Curt Connors/Lizard (voice)

Adrian Toomes/Vulture (voice)

Max Dillon/Electro (voice)

Montana/Shocker (voice)

J. Jonah Jameson (voice)

Flash Thompson (voice)

Otto Octavius/Doctor Octopus (voice)

Liz Allen (voice)

Sally Avril (voice), Betty Brant (voice)

Alex O'Hirn/Rhino (voice)