Peter Parker / Spider-Man (voice)

Mary Jane Watson (voice)

J. Jonah Jameson (voice)

Wilson Fisk / Kingpin (voice)

Felicia Hardy (voice)

Aunt May (voice)

Aunt May (voice)

Doctor Sylvia Lopez (voice)

Jason Phillips / Hobgoblin (voice)

Tombstone (voice)

Harry Osborn (voice)

Richard Fisk (voice)

Doctor Jonathan Ohn / The Spot (voice)

Norman Osborn / Green Goblin (voice)

Joe 'Robbie' Robertson (voice)

Landon (voice)

Lenny Luntz (voice)

Anna Watson (voice)

Ned Leeds (voice)

Doctor Curt Connors (voice)

Glory Grant (voice)

Jimmie Dugan (voice)

Debra Whitman (voice)

Stark (voice)

Flash Thompson (voice)

Madame Web (voice)

Martha Robertson (voice)

Nora (voice)

Randy Robertson (voice)

Joey Nails (voice)

Liz Allen (voice)